EquiChek™uses a novel competitive assay format to detect SAA in whole blood. The test delivers a semi-quantitative visual readout to help distinguish between normal and mild to clinically significant inflammation. The test is based on competition between SAA present in a sample and SAA printed onto the test strip with antibody coated gold nanoparticles. Unlike other tests EquiChek is not affected by the high dose hook effect.

• STEP 1
  Remove test cassette from foil pouch.
  
  
• STEP 2
  Remove cap from blood tube and insert sample applicator. Just touch sample applicator to surface of the blood. DO NOT IMMERSE SAMPLE APPLICATOR IN BL0OD TUBE. DO NOT SQUEEZE the sample applicator; blood will be drawn up automatically up to the red line on the sample applicator.
  
  
• STEP 3
  Apply sample to the sample port on the test strip. Touch sample applicator to the sample port and gently squeeze to expel blood into the test window.
  
  
• STEP 4
  Add 3 drops of liquid from the dropper bottle into the sample port.
  
  
• STEP 5
  The result can be seen in the Test Window (Figure 1). It is recommended that the test is read at between 5 - 10 minutes after starting. However, a normal result can be seen within 2-3 minutes, i.e. 4 lines will be visible. Do not read after 10 minutes as lines may fade.
  
  
• Interpretation of Results
  A normal healthy horse will have 4 clear lines in the test (see above).
  
  
• A horse with a strong inflammatory problem such as infection will have just 1 line visible.
  
  
• Where inflammatory status is borderline, 3 lines may be visible.
  
  
• Where there is mild to moderate inflammation, 2-3 lines will be visible.
  
  
• Horses with evidence of low grade inflammation should be retested within 24 hours to establish inflammatory status. As with all diagnostic tests, a definitive clinical diagnosis should not be based on a single result, but should only be made after all clinical and lab findings have been considered.
  
  

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